Fig. 9.

Temporal expression patterns of MrCTL mRNA in the hemocytes (A), stomach (B), and hepatopancreas (C) of M. rosenbergii challenged with V. harveyi. Total RNA was extracted from M. rosenbergii 6, 12, 24, and 48 h post-injection and profiled using qPCR. The mock control group of prawns received intramuscular injection (50 µL) of phosphate buffered saline (PBS, pH 7.4) and acted as mock (injected) control. β-actin (M. rosenbergii) was used as an internal control. Results of triplicate experiments are shown with standard errors. * P < 0.05, ** P < 0.01 (SAS, ANOVA)