Table 1.
Primers | Oligos name | Sequence (5′-3′) |
---|---|---|
Cloning | MrCTL-Fw | GCGACCATTCTTCTTCTTGC |
MrCTL-Rev | TTCTCTGAAGCGACTGCTTG | |
β-actin-Fw | AATCGTGCGTGACATCAAGG | |
β-actin-Rev | TCTCGTTACCGATGGTGATGAC | |
Sequencing | M13-Fw | GTAAAACGACGGCCAG |
M13-Rev | CAGGAAACAGCTATGAC | |
RACE-PCR | MrCTL (3′-RACE)-GSP1 | GTGAAGGGGAAGAAGACTGTATCC |
MrCTL (3′-RACE)-GSP2 | CAACCTGGGTTGGAACTTTG | |
MrCTL (5′-RACE)-GSP1 | GCAGGTTTCTCGCAGATGTA | |
MrCTL (5′-RACE)-GSP2 | GTCTTCTTCCCCTTCACAGTTG | |
UPM-Long | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | |
UPM-Short | CTAATACGACTCACTATAGGGC | |
qRT-PCR | MrCTL-Fw-qRT-PCR | GCATGACGGGACCTACAGAT |
MrCTL-Rev-qRT-PCR | ACTTTTCTGCAGGCGTTTGT | |
β-actin-Fw | GAGACCTTCAACACCCCAGC | |
β-actin-Rev | TAGGTGGTCTCGTGAATGCC |