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. 2022 Feb 5;30(2):1011–1035. doi: 10.1007/s10499-022-00845-3

Table 1.

Primers used for molecular cloning and expression analysis of MrCTL

Primers Oligos name Sequence (5′-3′)
Cloning MrCTL-Fw GCGACCATTCTTCTTCTTGC
MrCTL-Rev TTCTCTGAAGCGACTGCTTG
β-actin-Fw AATCGTGCGTGACATCAAGG
β-actin-Rev TCTCGTTACCGATGGTGATGAC
Sequencing M13-Fw GTAAAACGACGGCCAG
M13-Rev CAGGAAACAGCTATGAC
RACE-PCR MrCTL (3′-RACE)-GSP1 GTGAAGGGGAAGAAGACTGTATCC
MrCTL (3′-RACE)-GSP2 CAACCTGGGTTGGAACTTTG
MrCTL (5′-RACE)-GSP1 GCAGGTTTCTCGCAGATGTA
MrCTL (5′-RACE)-GSP2 GTCTTCTTCCCCTTCACAGTTG
UPM-Long CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT
UPM-Short CTAATACGACTCACTATAGGGC
qRT-PCR MrCTL-Fw-qRT-PCR GCATGACGGGACCTACAGAT
MrCTL-Rev-qRT-PCR ACTTTTCTGCAGGCGTTTGT
β-actin-Fw GAGACCTTCAACACCCCAGC
β-actin-Rev TAGGTGGTCTCGTGAATGCC