Table 2.
Primers used for molecular cloning and expression analysis of MrERGIC-53
| Primers | Oligos name | Sequence (5′-3′) |
|---|---|---|
| qPCR | MrERGIC-53-Fw | TCCGAATCACACCATCCTTAC |
| MrERGIC-53-Rev | GAAAGGCTTATTGCGGAAATC | |
| Sequencing | M13-Fw | GTAAAACGACGGCCAG |
| M13-Rev | CAGGAAACAGCTATGAC | |
| RACE-PCR | MrERGIC-53 (3′-RACE) GSP1 | GAGTGGTGGGAGGTAGACTTCGTTT |
| MrERGIC-53 (3′-RACE) GSP2 | AGGAGTTGAAGGTCCAGTCTTTGG | |
| MrERGIC-53 (5′-RACE) GSP1 | CCAAAGACTGGACCTTCAACTCCT | |
| MrERGIC-53 (5-RACE) GSP2 | AAACGAAGTCTACCTCCCACCACTC | |
| UPM Long | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | |
| UPM Short | CTAATACGACTCACTATAGGGC | |
| qRT-PCR | MrERGIC-53-Fw | TCCGAATCACACCATCCTTAC |
| MrERGIC-53-Rev | GAAAGGCTTATTGCGGAAATC | |
| β-actin-Fw | AATCGTGCGTGACATCAAGG | |
| β-actin-Rev | TCTCGTTACCGATGGTGATGAC |