Fig. 5.

Proteome dynamics associated with aging in healthy mice.A, average protein turnover in healthy mouse cortex at various ages. In total, 360 proteins were used to compare turnover across all ages (n = 3 at each postnatal age). p values for the comparisons (descending from top): 1.063e⁻⁰⁶, 4.557e⁻¹⁴, 1.220e⁻⁰⁵, 6.799e⁻¹⁴, 0.003, 0.175. B, average food consumption of mice at different ages: P113 (n = 4), P186 (n = 2), P285 (n = 4), P548 (n = 11). No significant change in appetite associated with aging was detected. p values for the comparisons (descending from top) = 0.127, 0.675, 0.047, 0.694, 0.011, 0.692. C, dynaplot of mouse cortex proteins depicting the change in proteome dynamics in healthy control mice from P113 to P186 (sky blue), to P285 (blue), and to P548 (dark blue). These data are expressed ratiometrically to allow simpler visualization between different ages of mice. D, bar graph showing apparent decrease in turnover of LC3, marker of autophagy, as mice age. Turnover was estimated by percentage heavy lysine incorporation (P6) in mice at postnatal days 113, 186, 285, and 503. ∗p < 0.05. n.s., non-significant.