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. 2021 Dec 18;21(2):100188. doi: 10.1016/j.mcpro.2021.100188

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 5 — The effect of ER inducers on AGR2–PDIA3 complex formation. Changes in subcellular localization of AGR2–PDIA3 complex in response to tunicamycin (TUN) and thapsigargin (THG) in comparison with untreated (control, CTR) (A) T47D and (B) A549 cells were analyzed using immunofluorescence staining for AGR2 (green), PDIA3 (red), and nucleus by DAPI (blue). The scale bar represents 10 μm. Colocalization of fluorescence signals was determined by Pearson's correlation coefficient, nonparametric one-way ANOVA (Kruskal–Wallis test with Dunn correction) test was used to calculate the statistical significance, ∗∗∗p ≤ 0.001. AGR2, anterior gradient 2; DAPI, 4′,6-diamidino-2-phenylindole; ER, endoplasmic reticulum; ns, nonsignificant; PDIA3, protein disulfide isomerase A3.