Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 3;41(6):865–877. doi: 10.1038/s41388-021-02133-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a PKM2 expression in BxPC-3/Capan-2 cells was modified by shRNA interference, and the expression of PKM2 and PD-L1 was measured by western blotting. b Tumor xenografts formed by implanted BxPC-3/Capan-2 cells with different expression levels of PKM2. c Scramble or sh-PKM2 was stably infected into BxPC-3/Capan-2cells, which were injected into nude mice. Tumor volumes were calculated after injection every 3 days. Tumor volumes are represented as the means of tumor volumes ±SEM (n = 10). d Tumor xenografts were isolated from the nude mice on day 24, the expression level of PD-L1 in tumor tissue was detected using western blotting. e Representative immunohistochemical staining of PKM2, PD-L1, and Ki-67 in tumor serial sections, which were derived from xenografts in the groups of BxPC-3/Capan-2 cells with different expression levels of PKM2. Scale bar, 50 μm. Red arrowheads showed PD-L1 or PKM2-positive tumor cells. f Single-cell suspensions from BxPC-3/Capan-2 tumors with different expression levels of PKM2 isolated from the nude mice on day 24 were stained for flow cytometry. Representative flow cytometry plots showed NK cells from BxPC-3 tumors with different expression levels of PKM2 (left), and the percentages of tumor-infiltrating leukocyte subsets (right) in gated CD45⁺ cells were analyzed from BxPC-3/Capan-2 tumors with different expression levels of PKM2 (n = 10 per group). g–i Single-cell suspensions from BxPC-3/Capan-2 tumors with different expression levels of PKM2 isolated from the nude mice on day 24 were stained for flow cytometry. Representative flow cytometry plots (left) and percentages of IFN-γ⁺, GzmB⁺, and NKp46⁺ NK cell (right) in gated CD3⁻ NK1.1⁺ cells (n = 10). Data are shown as mean ± SEM from five mice per group and are representative of two experiments. Statistical significance was determined by the Mann–Whitney U-test. NS = no significance; ^**P < 0.01.