Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 3;41(6):865–877. doi: 10.1038/s41388-021-02133-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Cytokine antibody arrays were performed in the culture media of M0/M2 macrophages co-cultured with BxPC-3 cells (left). The relative signal intensity of indicated cytokines in M2 macrophages co-cultured with BxPC-3 cells compared with M0 macrophages co-cultured BxPC-3 cells (right). b Quantitative RT-PCR analysis of mRNA levels of CXCL5, Thrombopoietin, IGF, EGF, and TGF-β1 in M0/M2 macrophages co-cultured with BxPC-3/Capan-2 cells for 48 h. c–g BxPC-3/Capan-2 cells were treated with or without TGF-β1 (20 ng/mL). c Quantitative RT-PCR analysis of mRNA expression levels of PD-L1 in BxPC-3/Capan-2 cells. d PKM2 and PD-L1 protein expression levels in BxPC-3/Capan-2 cells were determined using western blotting. e BxPC-3/Capan-2 cells were cross-linked by glutaraldehyde first and then subjected to western blotting. f Nuclear and cytosolic lysates were prepared from BxPC-3/Capan-2 cells, followed by western blotting. β-actin and histone H3 were used as loading controls. g The subcellular localization of PKM2 in BxPC-3/Capan-2 cells. Cells were immunostained with anti-PKM2 (PKM2, red). The nucleus was marked with 4′,6-diamidino-2-phenylindole dihydrochloride (blue). The stained cells were imaged using the ZEN 3.0 (Carl Zeiss) software. h BxPC-3/Capan-2 cells were treated with or without TGF-β1 (20 ng/mL), and P144 (10 μM) was used to block TGF-β1. Nuclear and cytosolic lysates were prepared from BxPC-3/Capan-2 cells, followed by western blotting. i Correlation analyses between the expression of TGF-β1 and PKM2/PD-L1 in PDAC tissues according to western blotting (n = 10). Data were shown as mean ± SEM from three experiments. Statistical significance was determined by a t-test; ^*P < 0.05,^**P < 0.01,^***P < 0.001.