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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2021 Oct 2;163:9–19. doi: 10.1016/j.yjmcc.2021.09.012

Figure 1. Tamoxifen induces depletion of Tip60 in hearts with floxed Kat5 alleles.

Figure 1.

Adult Kat5f/f and Kat5Δ/Δ mice were injected with 40 mg/kg tamoxifen on three consecutive days, after which hearts were collected and processed for assessment of Tip60 mRNA and protein. Panel A shows depletion of Kat5 mRNA in Kat5Δ/Δ hearts assessed by qRT-PCR 3–8 days after the first of three daily tamoxifen injections. Each biological replicate (n=4 mice for each timepoint per group) was subjected to three technical replicates. Panel B (upper) is a representative western blot showing depleted levels of Tip60 protein (55 kD) in Kat5Δ/Δ hearts collected 8–9 days after the first tamoxifen injection; the bar graph (lower) shows the extent of Tip60 depletion as assessed by quantitative densitometry. Each biological replicate (n=7 mice per group) was subjected to two technical replicates. Data are reported as Mean ± SEM and compared by unpaired, two-tailed Student’s t tests with Welch’s correction.