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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Cancer Immunol Res. 2021 Dec 22;10(2):215–227. doi: 10.1158/2326-6066.CIR-21-0772

Figure 1. Spatially informed analysis of immune cell populations in 907 colorectal cancer cases.

Figure 1.

A. Example multiplex immunofluorescence image for detection of GZMB, NCAM1, KRT, CD3, FCGR3A, PTPRC, and DAPI (nuclear stain). Scale bar: 100 μm. B-C. The image was processed with pathologist-supervised machine learning algorithms to identify tumor intraepithelial and stromal regions (B) and identify and classify each cell (C). In addition to the phenotypic criteria designated in the image, PTPRC expression was required for all immune populations. D-E. The distribution of densities of various immune cell populations in tumor intraepithelial (D) and stromal (E) regions. F. Spearman’s rank correlation coefficients between the densities of intraepithelial (IEL) and stromal (S) immune cells.