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. 2022 Feb 4;12:1941. doi: 10.1038/s41598-022-05781-z

Figure 2.

Figure 2

Diagrams of the relevant COI and Tpi gene segments, with descriptions of polymorphisms used to identify different haplotypes. Vertical lines in gene segments indicated site of single-base polymorphism with the thicker band indicating polymorphism diagnostic of strain identity. (A) COIB polymorphisms and the haplotypes observed in African FAW. Sites mCOI1164D and mCOI1287R identify strain and the h-haplotypes (h1-4). Sites mCOI1176 and mCOI1182 are also strain-specific in Western Hemisphere populations. (B) Diagram of the Tpi sequence used to PCR amplify the TpiE4 exon segment. Site gTpi183 is diagnostic of Tpi-based strain identity in all locations. Sites gTpi165 and gTpi168 are also strain-specific in the Western Hemisphere. Sites gTpi168 and gTpi180 are also strain-specific in Africa. Sites gTpi192 and gTpi198 are polymorphic but not strain-specific in all locations. Block arrows indicate location of primers used for PCR amplification and DNA sequencing.