Figure 6.

Zinc impairs Th2 house dust mite-induced asthmatic airway inflammation. (A) Heatmap showing Th2 genes of OT-II TCR tg CD4⁺ T cells in the absence and presence of 150 μM zinc. (B) MFI of GATA3 measured by FACS of OT-II TCR tg CD4⁺ T cells differentiated into Th1 and Th2 cells in the absence and presence of 100 μM zinc aspartate. (C) Frequencies of CD4⁺IL-13⁺ and CD4⁺IL-4⁺cells measured by FACS after Th1 and Th2 differentiation in the absence and presence of 100 μM zinc aspartate. (D) Mouse model of Th2 asthmatic airway inflammation induced by house dust mite (HDM) extract. (E) Frequencies of lung CD4⁺IL-13⁺ and CD4⁺IL-4⁺cells measured by FACS in the mouse model shown in Fig. 3D at day 14. (F) MFI of GATA3 in lung CD4⁺ T cells measured by FACS in the mouse model shown in Fig. 3D at day 14. (G) Peribronchial inflammation in the lungs from mice of the HDM asthma model shown in Fig. 3D ad day 14. (H) Total IgE serum levels of mice from the HDM asthma model shown in Fig. 3D ad day 14. RNA sequencing data in (A) was generated in biological triplicates from 3 mice. Statistical analysis in in (B) and (C) and (E–H) by unpaired student’s t test. Dots represent individual mice. Data are from 3 (B,C) and 2 (E–H) experiments. *p < 0.05, **p < 0.01, ***p < 0.001.