Skip to main content
. 2021 Aug 31;29(2):381–392. doi: 10.1038/s41418-021-00862-4

Fig. 1. p65 is a new substrate of FBXW2.

Fig. 1

Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies. A, B Immunoprecipitation (IP) and western blot analysis of the exogenous FBXW2/p65 proteins interaction in the 293T cells co-transfected with Flag-tagged FBXW2 and HA-tagged p65. C, D IP and western blot analysis of the endogenous FBXW2/p65 proteins interaction in the MCF-7 cells. E GST-pull down assay analysis of FBXW2/p65 proteins interaction using purified GST-tagged FBXW2 and His-p65. F Confocal immunofluorescence microscopy analysis of the p65/FBXW2 proteins interaction in the MCF-7 cells. G IP and western blot analysis of the HA-tagged p65/GFP-tagged FBXW2 fragments proteins interaction in the 293T cells. H IP and western blot analysis of the HA-tagged FBXW2/GFP-tagged p65 fragments proteins interaction in the 293T cells. I IP and western blot analysis of the HA-tagged p65/Flag-tagged FBXs proteins interaction in the 293T cells. J Evolutionary conservation of FBXW2 degron motif on p65. K IP and western blot analysis of the HA-tagged p65 (WT, 3D (S276D, D277D and S281D), 3 A (S276A, D277A and S281A), S276A or S276D)/Flag-tagged FBXW2 proteins interaction in the 293T cells.