Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 8;29(2):351–365. doi: 10.1038/s41418-021-00858-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A Hockey stick plots of the rank order of H3K27ac signals for all enhancers in BMSCs during adipogenic differentiation. Inserted panels of selected GO functional categories of SE-associated genes. The red arrow indicates the NEAT1-related SE. B Integrative Genomics Viewer (IGV) of H3K27ac-seq with DNase-seq and Med1-seq read density in NEAT1 of undifferentiated BMSCs and BMSCs with multilineages differentiation (OB osteogenic differentiation, AD adipogenic differentiation). C Promoter/enhancer reporter assays in young/aged BMSCs validated the age-related promoter/enhancer function of NEAT1. D NEAT1 expression level of BMSCs transfected with normal control or si-ATF2 (left panel) and control vector or ATF2 plasmid (right panel). E ChIP assays showed ATF2 binding sites in the NEAT1 promoter/enhancer region of young/aged BMSCs. F, G ATF2 knockdown and overexpression showed a significant influence on NEAT1 promoter/enhancer region activity. The results were presented as means ± S.D. *p < 0.05; **p < 0.01; ^#p > 0.05 by Student’s t test and one-way ANOVA.