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. 2021 Sep 8;29(2):351–365. doi: 10.1038/s41418-021-00858-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Western blotting revealed that osteogenesis was significantly attenuated in aged BMSCs, while adipogenesis was markedly strengthened. B–D Alizarin red staining, qRT-PCR, and western blotting showed that si-NEAT1 rescued the osteogenesis decline in aged BMSCs. E–G Alizarin red staining, qRT-PCR, and western blotting showed that NEAT1 overexpression decreased osteogenesis in young BMSCs. H–L Oil red O staining, qRT-PCR, and western blotting showed that NEAT1 promoted adipogenesis. Scale bar: 200 μm. The results were presented as means ± S.D. *p < 0.05; **p < 0.01; ***p < 0.001; ^#p > 0.05 by Student’s t test and one-way ANOVA.