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. 2022 Feb 5;14:57. doi: 10.1007/s40820-022-00801-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Cell viabilities of CL2 and Hepa1-6 cells (n = 4). b CLSM images of HMGB1 immunofluorescence staining. Scale bar = 20 µm. c Extracellular HMGB1 levels (n = 3) d CLSM images of CRT immunofluorescence staining. Scale bar = 20 µm. e Flow cytometry analysis of CRT immunofluorescence staining. f Extracellular ATP levels (n = 3). g Schematic illustration of the experimental design and procedure of DC maturation assay in vitro. h DC maturation assay by flow cytometry