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Estimation of intracellular GDP-fucose in adherent cells (a); in suspension cells (b). Enzymatically produced GN2M3FGN2-Asn-Fmocs were eluted by HPLCs. Negative control: Fut8+GN2M3GN2-Asn-Fmoc, positive control: GDP-Fuc+Fut8+GN2M3GN2-Asn-Fmoc, wild-type: CHO-K1 extracts+Fut8+GN2M3GN2-Asn-Fmoc, ΔGMD: ΔGMD extracts+Fut8+GN2M3GN2-Asn-Fmoc, ΔGMDΔGFT: ΔGMDΔGFT extracts+Fut8+GN2M3GN2-Asn-Fmoc
