Table 2.
N-glycan structures in suspension cells
| Structure | Wild-type | ΔGMD | ΔGMDΔGFT | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1st | 2nd | 3rd | 1st | 2nd | 3rd | 1st | 2nd | 3rd | |
| GLM9GN2 | 0.1 | 0.8 | 0.4 | 1.5 | 0.8 | 0.9 | 0.2 | 0.7 | 0.6 |
| M5-9GN2 | 73.4 | 57.7 | 61.8 | 78.0 | 53.1 | 63.8 | 75.4 | 71.6 | 50.9 |
| M6FGN2 | 0.1 | – | – | – | – | – | – | – | – |
| M5FGN2 | 0.4 | – | 0.2 | – | – | – | – | – | – |
| M4GN2 | 0.4 | 1.1 | 1.4 | 0.5 | 2.0 | 0.8 | 0.8 | 1.1 | 1.1 |
| M4FGN2 | – | – | 0.2 | – | – | – | – | – | – |
| M3GN2 | 0.3 | 1.2 | 1.2 | 1.1 | 9.1 | 5.1 | 1.6 | 4.9 | 5.3 |
| M3FGN2 | 0.7 | 4.7 | 3.4 | – | 0.1 | – | – | – | – |
| M2GN2 | – | – | – | – | 2.8 | 0.7 | – | – | 1.1 |
| M2FGN2 | – | 0.4 | 0.5 | – | – | – | – | – | – |
| GNM3GN2 | 0.1 | 0.9 | 0.1 | 0.6 | 7.1 | 5.4 | 0.5 | 3.0 | 5.0 |
| GNM3FGN2 | 0.4 | 3.6 | 3.9 | – | – | – | – | – | – |
| GNM5GN2 | 0.3 | 0.6 | 0.5 | 0.3 | 0.3 | 0.5 | 0.2 | 0.7 | 0.9 |
| GN2M3GN2 | – | 0.7 | 0.4 | 0.8 | 4.4 | 5.7 | 1.6 | 3.9 | 6.0 |
| GN2M3FGN2 | 0.4 | 2.3 | 3.3 | – | – | 0.1 | – | – | – |
| GN3M3GN2 | 0.1 | 0.3 | 0.1 | 0.3 | 1.5 | 2.7 | 0.5 | 0.9 | 2.3 |
| GN3M3FGN2 | 0.2 | 1.5 | 1.4 | – | – | – | – | – | – |
| GN4M3GN2 | – | – | – | 0.2 | 0.1 | 0.2 | 0.2 | 0.1 | 0.1 |
| GN4M3FGN2 | – | 0.1 | 0.1 | – | – | – | – | – | – |
| GN5M3GN2 | – | – | – | – | 0.1 | – | – | – | – |
| GalGNM3GN2 | – | 0.6 | 0.7 | 0.4 | 2.7 | 1.4 | 0.4 | 2.0 | 1.1 |
| GalGNM3FGN2 | 0.3 | 1.1 | 1.0 | – | – | – | – | – | – |
| GalGNM5GN2 | – | 0.4 | 0.1 | 0.4 | 0.2 | 0.1 | 0.3 | – | 0.3 |
| GalGN2M3GN2 | – | 0.1 | 0.1 | 1.3 | 2.1 | 0.7 | 1.0 | 3.5 | 1.5 |
| GalGN2M3FGN2 | 0.5 | 0.7 | 1.2 | – | – | – | – | – | – |
| GalGN3M3GN2 | 1.4 | 1.7 | 1.9 | 0.5 | 1.3 | 1.8 | 0.8 | 1.1 | 1.0 |
| GalGN3M3FGN2 | – | – | – | 0.1 | – | – | – | – | - |
| GalGN4M3GN2 | – | – | 0.1 | – | 0.1 | 0.1 | – | – | 0.4 |
| GalGNM6GN2 | – | – | – | – | – | 0.1 | – | – | – |
| Gal2GN2M3GN2 | 0.9 | 1.6 | 2.7 | 9.3 | 4.9 | 3.3 | 8.6 | 3.4 | 10.1 |
| Gal2GN2M3FGN2 | 3.7 | 7.5 | 2.8 | 0.2 | – | – | – | – | – |
| Gal2GN3M3GN2 | – | 0.1 | 0.3 | 0.3 | 0.9 | 0.6 | 0.4 | 0.8 | 0.6 |
| Gal2GN3M3FGN2 | 0.1 | – | 0.1 | – | – | – | – | – | – |
| Gal2GN4M3GN2 | – | – | – | – | 0.1 | – | – | – | – |
| Gal3GN3M3GN2 | 0.1 | 0.9 | 0.9 | 2.7 | 2.9 | 1.3 | 2.0 | 1.2 | 3.2 |
| Gal3GN3M3FGN2 | 1.4 | 1.6 | 1.4 | – | – | – | – | – | – |
| Gal3GN4M3GN2 | 0.3 | – | – | – | – | – | 0.2 | – | – |
| Gal3GN4M3FGN2 | 0.3 | – | – | – | – | – | – | – | – |
| Gal4GN4M3GN2 | – | – | – | 0.8 | 1.3 | 1.4 | 1.9 | 0.5 | 2.3 |
| Gal4GN4M3FGN2 | 2.0 | 1.7 | 1.3 | – | – | – | – | – | – |
| NAGalGNM3GN2 | 0.1 | – | 0.2 | – | – | – | 0.3 | 0.1 | 0.1 |
| NAGalGNM4GN2 | – | – | 0.2 | – | – | – | 0.2 | – | – |
| NAGalGNM5GN2 | 0.1 | 0.1 | – | – | – | – | 0.2 | – | 0.2 |
| NAGalGN2M3GN2 | 0.1 | 0.1 | – | 0.1 | – | – | – | – | 0.2 |
| NAGalGN2M3FGN2 | 0.3 | – | – | – | – | – | – | – | – |
| NAGalGN4M3GN2 | – | 0.1 | – | – | – | – | – | – | – |
| NAGal2GN2M3GN2 | 0.5 | 1.0 | 1.7 | 0.7 | 0.4 | 1.4 | 2.2 | 0.3 | 1.8 |
| NAGal2GN2M3FGN2 | 5.6 | 2.1 | 1.7 | – | – | – | – | – | – |
| NAGal2GN3M3GN2 | – | 0.4 | – | – | 0.1 | – | – | 0.1 | 0.1 |
| NAGal3GN3M3GN2 | – | - | - | 0.1 | 0.6 | 0.6 | 0.6 | 0.1 | 1.9 |
| NAGal3GN3M3FGN2 | 1.4 | 1.2 | 1.4 | – | – | – | – | – | – |
| NAGal4GN4M3GN2 | – | – | – | – | 0.2 | 0.3 | – | – | 0.9 |
| NGGalGN2M3GN2 | – | – | – | – | 0.3 | 0.2 | 0.1 | – | 0.1 |
| NGGal2GN2M3GN2 | – | – | – | – | 0.3 | 0.2 | 0.3 | – | 0.1 |
| NGGal2GN2M3FGN2 | – | 0.2 | – | – | – | – | – | – | – |
| NGGal3GN3M3GN2 | – | – | – | – | 0.1 | – | – | – | –– |
| NA2Gal2GN2M3GN2 | 1.1 | 0.2 | – | – | 0.3 | 0.6 | 1.1 | – | 0.9 |
| NA2Gal2GN2M3FGN2 | 2.9 | 0.8 | 1.1 | – | – | – | – | – | – |
Analysis was conducted three times for each cell line (1st, 2nd, 3rd)
The italicized area shows the data of fucosylated glycans