Figure 5.

NPY-mediated inhibition of estradiol-stimulated cell growth is reversed by NPY1R antagonist BIBP-3226 in ER+ BC cells. MCF7 and T47D cells were treated with either vehicle (0.1% DMSO) (A, B) or 1 µM BIBP-3226 (C, D) and then plated in 96-well plates. After overnight attachments, the cells were treated with either vehicle (0.1% ethanol), 100 nM neuropeptide Y, 1 nM estradiol, or 100 nM NPY + 1 nM estradiol. The cell proliferation was determined using MTT assay after 72 h. *indicates p < 0.05, One-way ANOVA, Tukey test.