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. 2021 Aug 30;29(2):323–336. doi: 10.1038/s41418-021-00855-3

Fig. 4. Bmf T72E mutation promotes mitochondria translocation.

Fig. 4

A MCF-7 HA-Bmf (WT/T72E) or MCF-10A HA-Bmf (WT/T72E) cells were induced with doxycycline (100 ng/mL) for 4 h. Myosin V-rich P1 fraction and mitochondria fraction were separately isolated and analyzed by western blotting. B MCF-7 HA-Bmf (WT/T72E) cells was induced by doxycycline for 1 h and immunofluorescence staining of HA-Bmf (green) and mitochondria (red, labeled by mitotracker) was performed. Scale bar = 20 μm.