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. 2022 Feb 4;13(2):114. doi: 10.1038/s41419-022-04572-8

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© The Author(s) 2022, corrected publication 2022

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Fig. 1 — Seahorse XFe96 bioanalyzer was used to measure oxygen-consumption rate (OCR) in Panc1 (A) and Aspc1 cell lines (B) either untreated (0) or pretreated with 0.4, 0.8, 1.6, 3.125, 6.25, 12.5, or 25 μM AdipoRon for 45 min, with initial basal measurements followed by sequential injections of oligomycin, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), and rotenone/antimycin A. Mitochondrial uncoupling (C, D), basal respiration (E, F), maximal respiration, (G, H), ATP production (I, J), and proton leak (K, L) were calculated from the OCR measurements in (A, B respectively). One-way ANOVA, n ≥ 3; error bars, SD; *P < 0.05.