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. 2022 Feb 4;13(2):114. doi: 10.1038/s41419-022-04572-8

Fig. 2. AdipoRon inhibits mitochondrial respiration and ATP production in pancreatic cancer cells.

Fig. 2

Seahorse XFe96 bioanalyzer was used to measure oxygen-consumption rate (OCR) in six different PDAC lines (A) either untreated or pretreated with 25 μM AdipoRon for 45 min, with initial basal measurements followed by sequential injections of oligomycin, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), and antimycin A and rotenone. Basal respiration (B), maximal respiration (C), ATP production (D), proton leak (E), and mitochondrial uncoupling (F) were calculated from the OCR measurements in (A) for the respective cell lines. Statistics: t-test, n ≥ 3; error bars, SD; *P < 0.05.