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. 2022 Feb 4;13(2):114. doi: 10.1038/s41419-022-04572-8

Fig. 3. AdipoRon activates glycolysis in pancreatic cancer cell lines.

Fig. 3

Seahorse XFe96 bioanalyzer was used to measure extracellular acidification rate (ECAR) in six different PDAC lines (A) either untreated (vehicle) or pretreated with 25 μM AdipoRon for 45 min, with initial basal measurements followed by sequential injections of glucose (10 mM), oligomycin (2 µM), and 2-deoxy-d-glucose (2-DG) (50 mM). Glycolysis (B), glycolytic capacity (C), and glycolytic reserve (D) were calculated from the ECAR measurements in (A) for the respective cell lines. Statistics: t-test, n ≥ 3; error bars, SD; *P < 0.05.