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. 2021 Sep 11;29(2):439–450. doi: 10.1038/s41418-021-00867-z

Fig. 7. TRIM21 deficiency ameliorates LPS-induced inflammation and DSS-induced colitis.

Fig. 7

a Kaplan–Meier survival plot for WT (n = 10), Trim21−/− (n = 7), and Gsdmd−/− mice (n = 7) intraperitoneally injected with PBS or LPS (50 mg/kg). b IL-1β and IL-18 concentrations in the serum from WT, Trim21−/− and Gsdmd−/− mice. c–e Representative images of immunoblot analysis of the indicated proteins in soluble and insoluble fractions of kidney and lung tissues from WT, Trim21−/−, and Gsdmd−/− mice (d, e). The relative concentration of GSDMD-N in LPS-injected groups was quantified by densitometry, and the results were shown in (c). f Kaplan–Meier survival plot for WT and Trim21−/− mice (n = 5 mice per group) given 5% DSS in their drinking water for 7 days, followed by regular drinking water. g Body weights of mice treated as in (a) were recorded on different days. h, i, Mice were killed on day 8. The macroscopic appearance (h) and colon lengths (i) of the mice were measured. j Histopathological changes in colon tissues were examined by H&E staining. Black arrows represent vascular rupture. Scale bar, 50 μm. k Immunohistochemistry (IHC) analysis of Ki67 expression in normal and Trim21−/− colon tissues. Scale bar, 100 μm. l GSDMD and TRIM21 protein expression levels in colon tissues were analyzed by immunoblotting. The data are the means ± SD of triplicate samples from a representative experiment. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. All data are representative of three independent experiments.