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. 2022 Feb 1;28(5):669–677. doi: 10.1016/j.jiac.2022.01.019

Fig. 1.

Fig. 1

(A) Location of SARS-CoV-2 genome where the RT-qPCR primer-probe sets target for detecting SARS-CoV-2 in patient samples. Both primer-probe sets (N1 and N2) target the N protein-coding sequences. N1_F, N1_R, N1_P are the primers and probe of the N1 set and N2_F, N2_R. N2_P are the primers and probe of the N2 set (Figure not drawn to scale). (B) RT-qPCR curve to detect SARS-CoV-2 using NIID recommended N2 primer-probe set produced aberrant curve in two samples (A8167 and A8168) compared to other positive samples in the same run.