FIGURE 1.

Number of dedifferentiated islet β cells in diabetic patients and diabetic mice is remarkably increased. (A) The paraffin sections of pancreas from patients with and without diabetes mellitus were stained with immunofluorescence double staining, red stands for insulin, green for SOX9 and blue for DAPI. Bar = 100 μm. (B) The area ratio of SOX9 to insulin in islets of control and diabetic patients (n = 3). (C) The results of immunofluorescence double staining of paraffin sections of pancreas tissues of db/m and db/db mice, red is Insulin, green is SOX9 and blue is DAPI. Bar = 100 μm. (D) The area ratio of SOX9 to insulin in islets of db/m and db/db mice (n = 3). (E) Western blot results and relative expression value results (F) of pancreatic tissue proteins SOX9, NGN3 in control and diabetic patients (n = 3). (G) Western blot results and relative expression value results (H) of pancreatic tissue proteins SOX9, NGN3 in db/m and db/db mice (n = 3). *p < 0.05,**p < 0.01. ***p < 0.001