FIGURE 2.

SDF‐1 is significantly related to the dedifferentiation of islet β cells. (A) The paraffin sections of pancreas from patients with and without diabetes mellitus were stained with immunofluorescence double staining, red stands for insulin, green for SDF‐1 and blue for DAPI. Bar = 100 μm. (B) The area ratio of SDF‐1 to insulin in islets of control and diabetic patients (n = 3). (C) Paraffin sections of pancreatic tissues of db/m and db/db mice were double‐stained with insulin and SDF‐1 by immunofluorescence. Red stands for insulin, green for SDF‐1 and blue for DAPI. Bar = 100 μm. (D) The area ratio of SDF‐1 to insulin in islets of db/m and db/db mice (n = 3). (E) The paraffin sections of pancreatic tissues of diabetic patients and non‐diabetic patients were stained by immunofluorescence double staining, green stands for SDF‐1, red for SOX9 and blue for DAPI. Bar = 100 μm. (F) The area ratio of SDF‐1 to SOX9 in islets of control and diabetic patients. (n = 3). (G) The immunofluorescence of paraffin sections of pancreas of db/m and db/db mice showed that red stands for SDF‐1, green for SOX9 and blue for DAPI. Bar = 100 μm. (H) The area ratio of SDF‐1 to SOX9 in islets of db/m and db/db mice (n = 3). (I) Pancreatic tissue Western blot results and relative expression value results (J) of non‐diabetic patients and diabetic patients (n = 3). (K) The results of Western blot and relative expression value results (L) of pancreatic tissue in db / m and db / db mice (n = 3). **p < 0.01,***p < 0.001