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. 2021 Dec 21;26(3):750–763. doi: 10.1111/jcmm.17110

FIGURE 4.

FIGURE 4

SDF‐1 can phosphorylate AKT, then upregulated the expression of FoxO1 and finally inhibited the dedifferentiation of islet β cells. (A) Western blot results of human and mouse pancreatic tissue proteins under non‐diabetic and diabetic conditions, and the relative expression value results (B) (n = 3). (C) In dedifferentiated Min6 cells, the Western blot results and the relative expression value results (D) after inhibiting the expression of SDF‐1 by siRNA transfection (n = 3). (E) In dedifferentiated Min6 cells, Western blot results and relative expression value results (F) after plasmid transfection to overexpress SDF‐1 (n = 3). (G) Immunofluorescence staining results of pancreatic tissue sections of non‐diabetic patients and diabetic patients. Bar = 100 μm. (H) The area ratio of SDF‐1/CXCR4 to insulin in islets of control and diabetic patients (n = 3). (I) Western blot and relative expression value results (J) of pancreatic tissue proteins in non‐diabetic and diabetic patients. (K) The results of immunofluorescence staining of pancreatic tissue sections of db/m and db/db mice. Bar = 100 μm. (L) The area ratio of SDF‐1/CXCR4 to insulin in islets of db/m and db/db mice (n = 3). (M) Western blot results and relative expression value results (N) of pancreas tissues of db/m and db/db mice (n = 3). *p < 0.005,**p < 0.01,***p < 0.001