Androgen/AR signalling increases ZFHX3 expression at both mRNA and protein levels in PCa cells. (A–E) Detection of ZFHX3 protein and ZFHX3 mRNA by western blotting and real‐time qPCR, respectively, along with AR and α‐tubulin as controls in western blotting and KLK3 as a control in qPCR, in LNCaP (A, C) and C4‐2B (B, D, E) cells cultured in the hormone‐free medium for 24 h and then treated with R1881 at indicated concentrations for 24 h (A, B, E) or 1 nM for indicated times (C, D), with (E) and without the knockdown of AR (A–D). Transfection of siRNAs was for 24 h before R1881 treatment. siCtrl, control siRNA; siAR, AR siRNA. (F) In C4‐2B cells cultured in complete medium supplemented with R1881 at 1 nM and enzalutamide at indicated concentrations for 24 h, expression of ZFHX3, AR, α‐tubulin, and KLK3 was detected as in panels A‐D. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001