TABLE 2.
ELISA results for sera from patients and random donors
| ELISA and immunoglobulina | Sensitivityb
|
Specificityc | |
|---|---|---|---|
| Acute-phase sera | Convalescent-phase sera | ||
| Erf-1 | |||
| IgG | 14/27 (51.8) | 25/27 (92.6) | 97.2 (1/36) |
| IgM | 15/27 (55.6) | 23/27 (85.2) | 100 (0/36) |
| IgG + IgM | 15/27 (55.6) | 25/27 (92.6) | 97.2 (1/36) |
| HGE-9 | |||
| IgG | 18/27 (66.7) | 19/27 (70.4) | 97.3 (1/37) |
| IgM | 12/27 (44.4) | 18/27 (66.7) | 100 (0/37) |
| IgG + IgM | 20/27 (74.1) | 20/27 (74.1) | 97.3 (1/37) |
| Erf-1 + HGE-9 | |||
| IgG | 19/27 (70.4) | 25/27 (92.6) | |
| IgM | 16/27 (59.2) | 23/27 (85.2) | |
| IgG + IgM | 21/27 (77.8) | 25/27 (92.6) | |
| HGE-2 | |||
| IgG | 15/27 (55.6) | 21/27 (77.8) | 97.3 (1/37) |
| IgM | 4/27 (14.8) | 3/27 (11.1) | 94.6 (2/37) |
| IgG + IgM | 15/27 (55.6) | 21/27 (77.8) | 91.9 (3/37) |
| HGE-14 | |||
| IgG | 13/27 (48.1) | 13/27 (48.1) | 96.8 (1/31) |
| IgM | 8/27 (29.6) | 7/27 (25.9) | 93.5 (2/31) |
| IgG + IgM | 14/27 (51.8) | 13/27 (48.1) | 93.5 (2/31) |
| HGE-15 | |||
| IgG | 12/27 (44.4) | 17/27 (63.0) | 97.3 (1/37) |
| IgM | 12/27 (44.4) | 14/27 (51.8) | 97.3 (1/37) |
| IgG + IgM | 13/27 (48.1) | 19/27 (70.4) | 94.6 (2/37) |
| HGE-17 | |||
| IgG | 12/27 (44.4) | 14/27 (51.8) | 94.6 (2/37) |
| IgM | 14/27 (51.8) | 15/27 (55.6) | 100 (0/37) |
| IgG + IgM | 15/27 (55.6) | 19/27 (70.4) | 94.6 (2/37) |
| Total (all antigens) | |||
| IgG | 21/27 (77.8) | 26/27 (96.3) | |
| IgM | 16/27 (59.2) | 22/27 (81.5) | |
| IgG + IgM | 23/27 (85.2) | 26/27 (96.2) | |
| IFA (≥64) (IgG + IgM) | |||
| Erf-1 + HGE-9 | 15/17 (88.2) | 23/25 (92.0) | |
| All antigens | 16/17 (94.1) | 24/25 (96.0) | |
a
The recombinant protein identity and the secondary antibody used in the assay are indicated.
b
Results for sensitivity are number of samples with positive reactions/total number of samples tested (percent positive results). Cutoff values were determined from the mean value plus 3 standard deviations of the mean for samples from random donors from areas of nonendemicity. Mean assay values with standard errors of the mean for each patient group tested are shown in Figure 5.
c
Results for specificity are percent positive results (number of samples with positive reactions/total number of samples tested).