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. 2022 Feb 5;214:118162. doi: 10.1016/j.watres.2022.118162

Fig. 2.

Fig. 2

Amino Acid Mutations detected in wastewater samples. (Left) Allele frequency of the mutations (relative to the reference genome Wuhan-Hu-1 [GenBank accession numbers NC_045512 and MN908947.3]), associated with the variant of concern (VOCs), in each wastewater sample. (Middle) Number of mutations (count) detected in each sample corresponding to each VOC. (Right) Heatmap showing the presence (green) / absence (white) of mutations having more than 50% allele frequency. AA mutation: Amino acid mutations; AF: Allele Frequency; S: spike Protein; ORF8: open reading frame 8; ORF3a: open reading frame 3a; and N: nucleocapsid protein.