TABLE 1.
Detection of Salmonella using cultivation, PCR-hybridization, and a combination of cultivation and PCR-hybridization following enrichment using three different media
| Farm no. and sample type (no. of samples) | Enrichment medium (-a) | Detection (%) of Salmonella (no. positive/total no. of samples) by method:
|
||
|---|---|---|---|---|
| Cultivation | PCR-based detection | Cultivation–PCR-based detection | ||
| Farm 1, feces (29) | TSB-RV10 | 0.0 | 0.0 | 0.0 |
| Farm 2, feces (22) | TSB-RV10 | 0.0 | 0.0 | 0.0 |
| Farm 3, feces (41) | TSB-RV10 | 53.7 (22/41) | 48.8 (20/41) | 60.9 (25/41) |
| Total | 23.9 (22/92) | 21.7 (20/92) | 27.2 (25/92) | |
| Farm 3, water (24) | 3MC-RV10 | 58.3 (14/24) | 50.0 (12/24) | 62.5 (15/24) |
| Farm 4, feces (34) | RV10 | 32.4 (11/34) | 58.8 (20/34) | 73.5 (25/34) |