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Southern blot analysis of mtb48 showing hybridization of a labeled tbh4 nucleotide probe with PstI-digested genomic DNA from the M. tuberculosis and M. bovis BCG isolates tested but not from M. leprae or the environmental mycobacteria tested. Arrows indicate the migration of a labeled 6.5-kbp fragment from the BCG strain of M. bovis (top arrow) and 5.5-kbp DNA fragments from M. tuberculosis isolates (bottom arrow).
