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. Author manuscript; available in PMC: 2022 Feb 5.
Published in final edited form as: J Proteomics. 2018 Sep 21;192:233–245. doi: 10.1016/j.jprot.2018.09.005

Figure 3. Quantification of Heme Oxygenase 1 (HO1) by Mass Spectrometry and Western blot:

Figure 3

(A) Bar diagrams showing the average reporter ion counts for HO1 following MS/MS analyses. (B) Aliquots of crude membrane extracts (40 μg) from control and 2HF (50μM, 24h) treated MCF10a, MCF7, MDA-MB231, and SKBR3 cells were applied to SDS-PAGE and subjected to Western blot analyses against anti-HO1 IgG. Numbers below the blots represent the fold change in the levels of proteins as compared to control as determined by scanning densitometry. β-actin was used as an internal control. C, control; T, 50 μM 2HF treated.