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. Author manuscript; available in PMC: 2022 Feb 5.
Published in final edited form as: J Proteomics. 2018 Sep 21;192:233–245. doi: 10.1016/j.jprot.2018.09.005

Figure 7. Effect of 2HF (50 μM for 24h) on HSP90, DNMT1, PTEN, and cMyc expression in breast cancer cells:

Figure 7

Aliquots of crude membrane extracts (40 μg) from control and 2HF (50μM, 24h) treated MCF7, MDA-MB231, and SKBR3 cells were applied to SDS-PAGE and subjected to Western blot analyses against HSP90, DNMT1, PTEN, and cMyc antibodies. Numbers below the blots represent the fold change in the levels of proteins as compared to control as determined by scanning densitometry. β-actin was used as an internal control. C, control; T, 50 μM 2HF treated.