Table 3.
Mass (M + 1) | Retention time (min) | Description | Relative presence | |
---|---|---|---|---|
RPQ | SPQ | |||
246.1585 | 4.3 | Demethylated primaquine | + | + |
260.1408 | 1.87 | Primaquine-o-quinone | + | + |
261.1609 | 8.7 | Oxidative deamination to primaquine alcohol | ++ | + |
274.1544 | 2.35 | Hydroxylation and quinone-imine formation | + | − |
276.1690 | 4.18 | 2-Hydroxy-primaquine | − | + |
276.1691 | 4.88 | 3-Hydroxy-primaquine | + | − |
289.1531 | 10.04 | CarboxyPQ o-quinone | + | − |
290.1510 | 3.45 | Dihydroxy-PQ quinone imine | + | − |
332.1604 | 7.7 | Acetylated dihydroxy-PQ quinone-imine | ++ | + |
422.1929 | 2.3 | Demethylation + glucuronidation | + | + |
422.2266 | 4.9 | Glycosylated primaquine | + | + |
452.2027 | 1.9 | Hydroxy-primaquine glucuronide | − | + |
452.2027 | 2.37 | Hydroxy-primaquine glucuronide | + | − |
452.2027 | 3.4 | Hydroxy-primaquine glucuronide | + | + |
452.2027 | 4.3 | Hydroxy-primaquine glucuronide | − | + |
467.1642 | 5.6 | Glucuronide of hydroxylated carboxyprimaquine | − | + |
467.1642 | 7.27 | Glucuronide of hydroxylated carboxyprimaquine | + | − |
480.1989 | 7.9 | Primaquine-N-carbamoyl glucuronide | + | + |
494.2125 | 3.0 | Primaquine acetylation, hydroxylation and glucuronidation | + | − |
494.2125 | 2.9 | Primaquine acetylation, hydroxylation and glucuronidation | − | + |
494.2125 | 4.7 | Primaquine acetylation, hydroxylation and glucuronidation | − | + |
(−) denotes the absence of the metabolite. Where present in both enantiomers, relative amount is denoted with (+) and (++) denoting low and high amounts respectively