Cassettes used for the simplified protocol (numbered 1–6). 1, Cerebellum; basal ganglia; 2, hippocampus at lateral geniculate nucleus (LGN); superior frontal cortex (Brodmann Area [BA] 8, 9); 3, midbrain; cingulate gyrus (BA24); 4, medulla oblongata; pons; 5, calcarine cortex (BA17); superior frontal cortex (BA7); 6, thalamus; temporal pole (BA38). See Table 1 for staining protocol.