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. 2022 Feb 5;19:26. doi: 10.1186/s12985-022-01755-9

Fig. 3.

Fig. 3

Schematic diagram of AH/17 and FJ/20 chimeras and mutants. A Amino acid substitutions in four chimeric viruses. Four types of chimeras were divided and grouped according to the position of different amino acids on the surface of HA protein. B Schematic illustration of the rescued chimeras and mutants by reverse genetics. Amino acid substitutions were introduced into the AH/17 and FJ/20 HA genes by PCR-based site-directed mutagenesis and viruses were generated by 6:2 reverse genetics system