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. Author manuscript; available in PMC: 2022 Feb 5.
Published in final edited form as: Ticks Tick Borne Dis. 2020 Jan 20;11(3):101377. doi: 10.1016/j.ttbdis.2020.101377

Table 1.

Primers used for the construction of the OspC complementation plasmid.

Primer Name Sequencea Ref
ReconF+XmaI GTGCCCGGGGATTATAAGATTTAACAC (Battisti et al., 2008)
vtpP3’-NdeI CTTCTTCATATGTATGTGCCTCCTTATTAACATACATTAATAGTGC This work
OspC5’-NdeI GCACAAACATATGAAAAAGAATACATTAAGTGC This work
OspC3’-BamHI/NcoI GGATCCATGGTTAAGGTTTTTTTGGACTTTCTGCCACAAC This work
Gent5’+XmaI ATTCGCCCGGGCCGGCAATTCCTAATCAGAAAAATGTGG (Battisti et al., 2008)
pOKvtpF-NcoI AACCATGGTATATATAATTTTAAATATTATTATAAG This work
BBB12+ CTTGTCATTCCTTTACCAACCG This work
a

Bases in bold indicate nuclease restriction sites