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. Author manuscript; available in PMC: 2022 Feb 5.
Published in final edited form as: J Vis Exp. 2019 Jun 25;(148):10.3791/59926. doi: 10.3791/59926

Figure 3. Inclusion of secretion modulating compounds during glucose-stimulates InsGLuc secretion.

Figure 3.

InsGLuc MIN6 cells plated in 96 well format as described were preincubated in glucose-free KRBH for 1 h. Cells were then treated with or without 20 mM glucose in the presence of DMSO (0.1%), KCl (35 mM), diazoxide (250 μM), PMA (100 nM), or epinephrine (1 μM) for 1 h. Bar graph is the mean ± SE of at least 3 independent experiments.