FIG. 2.

Application of the SplintR qPCR assay to quantify chemically and structurally diverse oligonucleotides. For quantification, each ASO was dissolved in water. The ASOs detected and quantified include (A) fully modified ASOs, (B) LNA gapmer, and (C) siRNA. Full 2′-O-MOE ASO used 25 U/reaction of SplintR ligase, while other ASOs used 2.5 U/reaction. The qPCR for each standard point was performed in technical triplicate. The standard curve was plotted as Cq value vs. the log of ASO concentration in the sample. (D) List of linear ranges of quantification and coefficients of determination (R²) for all the ASOs reported in this study.