. 2021 Autumn;14(Suppl1):S32–S40.

Table 1.

List of primers and cycling program of p53 gene (exons 5, 6 and 8-11).

Region (PCR Product)	Table 1	Sequence (5'>3')	Cycle*	[MgCl₂]
Exon 5&6 (845 bp)	Forward	CTGATAGGCCATGGGTGAGT	94ºC(35 s), 63.2ºC (40 s),72ºC (45 s)	1.5mM
Exon 5&6 (845 bp)	Reverse	CTACGCTGAGGGAAACCTTG	94ºC(35 s), 63.2ºC (40 s),72ºC (45 s)	1.5mM
Exon 8 (676 bp)	Forward	GTTGACCTGGTCCTTTGAG	94ºC ( 35s), 55.4ºC (40 s),72ºC (45 s)	1.2 mM
Exon 8 (676 bp)	Reverse	CCGACAATTAAGATGGAGTG	94ºC ( 35s), 55.4ºC (40 s),72ºC (45 s)	1.2 mM
Exon 9 (483 bp)	Forward	TCATACTACATGCTCCTGACAC	94ºC ( 30s), 59.8ºC (30 s), 72ºC ( 45 s)	1.4mM
Exon 9 (483 bp)	Reverse	TTTCCATTCCTTCCACCCAG	94ºC ( 30s), 59.8ºC (30 s), 72ºC ( 45 s)	1.4mM
Exon 10 (580 bp)	Forward	GACATGATCTTCTTGGTGAGC	94ºC ( 30s), 58.2ºC (35 s), 72ºC ( 40 s)	1.4mM
Exon 10 (580 bp)	Reverse	ATCCCCTTTCTCCTTCATCC	94ºC ( 30s), 58.2ºC (35 s), 72ºC ( 40 s)	1.4mM
Exon 11 (650 bp)	Forward	ACTTCTTGGCACTTTAGCAGAG	94ºC (35 s), 52.9ºC (35 s), 72ºC (45 s)	1.2 mM
Exon 11 (650 bp)	Reverse	GGGCTAAATTTTCTAGCACTGG	94ºC (35 s), 52.9ºC (35 s), 72ºC (45 s)	1.2 mM

*Considering that for all reactions, the initial denaturation and final extension were 5 minutes at 94^oC and 72 ^oC for 10 minutes respectively