Normal colonic mucosa exhibits cytoplasmic expression of Das-1 (A), while normal small intestinal mucosa is negative for Das-1 (B). Similarly, normal pancreatic parenchyma including pancreatic ducts are non-reactive to Das-1 (C: H&E stain, D: Das-1 immunohistochemistry). Interestingly, rare small foci with acinar to ductal metaplasia in the setting of chronic pancreatitis secondary to pancreatic ductal adenocarcinoma shows strong membranous and/or cytoplasmic Das-1 expression (E: H&E stain, F: Das-1 immunohistochemistry).