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. 2022 Jan 29;2022:1755563. doi: 10.1155/2022/1755563

Figure 6.

Figure 6

VDR positively regulates the expression of Sirt1 through binding to the VDRE in the SIRT1 promoter. (a) Luciferase reporter assay confirms that overexpression of VDR positively regulates the mRNA expression of Sirt1. ∗∗P < 0.01. Blue bar: vector group; red bar: VDR group. (b) ChIP-qPCR detection verifies a direct binding between VDR and Sirt1 DNA promoter. Blue bar: vector group; red bar: VDR group. (c) Three specific mutation plasmids were constructed, and the results of luciferase reporting assays show that the third binding region in RXR is not functional, indicating the efficient binding relationship just exists between VDR and Sirt1 promoter. The plasmids of Luc-SIRT1 and VDR overexpression plasmid or VDR siRNA were transfected into 293T cells so were the Luc-Sirt1-mut1, Luc-Sirt1-mut2, and Luc-Sirt1-mut3 plasmids with VDR overexpression plasmid. ∗∗P < 0.01; ns: not significant; using Student's t-test. The experiments were repeated more than 3 times. Sirt1-mut1: transfected with Luc-Sirt1-mut1; Sirt1-mut2: transfected with Luc-Sirt1-mut2; Sirt1-mut1: transfected with Luc-Sirt1-mut3; blue bar: IgG group; red bar: VDR group.