Fig. 1.

Modified nucleosides synthesized in both natural and isotopically labeled forms for parallel quantification study. The modified nucleosides, ms²i⁶A, m⁶A, m¹G, t⁶A, i⁶A, m²A are present at position 37; m²G is present at position 9; m²₂G is present at positions 24 and 25; Am present at position 4; Q is present at the wobble position as well as Gm, which is additionally present at position 18; m¹A is present at positions 14 and 58 (mainly); Cm is present at position 32; m⁵C is present at position 38; m⁷G is present at position 46; m⁶₂A is present at position 37 (Brandmayr et al. 2012; Duechler et al. 2016)