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. Author manuscript; available in PMC: 2022 Jun 20.
Published in final edited form as: Nat Chem. 2021 Dec 20;14(2):196–207. doi: 10.1038/s41557-021-00840-w

Extended Data Figure 7: Examining the effects of other spacer residues on A1-LCD phase behavior.

Extended Data Figure 7:

(a) Diagram of variants to understand the contributions of Asn, Gln and Thr to effective solvation volumes of A1-LCD. In variant −14N-4Q+18G, the role of Asn and Gln residues in comparison to Gly spacers is assessed. In variants −14N+14Q and −23S+23T, residues with similar intrinsic free energies of solvation but different steric bulk are substituted. Vertical bars in the schematics indicate the position of residue types, namely Asn (red), Gln (yellow), Gly (green), Ser (black) and Thr (purple). (b) Measured binodals of A1-LCD variants from (a) as a function of temperature. (c) A focused view on the dilute arms (saturation concentrations) of the binodals in (b). The solution conditions for all experiments were 20 mM HEPES, 150 mM NaCl, pH 7.0.