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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Cell Stem Cell. 2022 Jan 21;29(2):232–247.e7. doi: 10.1016/j.stem.2021.12.009

Figure 7. Limiting iron availability stimulates HSC self-renewal and expansion ex vivo.

Figure 7.

(A–B) Total cell and CD150+ HSC numbers in the PVA-based culture system with defined transferrin levels (n = 7–8).

(C–D) Lin− cell ratio, cell cycling and ROS production of HSCs in the PVA-based culture system with defined transferrin levels (n = 4–8).

(E) Transplantation analysis of HSCs expanded with 500 mg/L or 0.5 mg/L transferrin (n = 4–5).

(F) Model figure showing a Goldilocks zone for HSC ex vivo expansion, with higher or lower iron levels impairing HSC self-renewal.

* p < 0.05, ** p < 0.01, *** p < 0.001. Error bars, mean ± SEM.