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. Author manuscript; available in PMC: 2022 May 29.
Published in final edited form as: Nat Cancer. 2021 Nov 29;3(1):43–59. doi: 10.1038/s43018-021-00279-5

Fig. 6. MTDH-SND1 complex disruption suppresses breast cancer progression and metastasis.

Fig. 6

a, Schematic diagram of the treatments in FVB female mice. b,c, Tumor size (b) and mass was determined(c). Vehicle, n=10 mice, C26-A6, n=12 mice. Size bar, 2 cm. d, H&E staining was performed with lungs, and metastatic nodules were counted. The metastatic nodules of the representative lungs were highlighted with red and blue respectively. Vehicle, n=10 lungs; C26-A6, n=12 lungs. Size bar, 5 mm. e, Gene set enrichment analysis plot showing the enrichment of Tmx treatment-upregulated (left), -downregulated (middle), or SND1-upregulated (right) gene signatures. p and q values were determined by Kolmogorov-Smirnov statistic with GSEA v3.0. f-h, PyMT;UBC-CreERT+/−;Mtdhfl/fl tumor cells with 4-OHT pre-treated were assessed by western blot (f). 10k of the cells were inoculated into FVB female mice and treated with or without C26-A6after primary tumors reached to ~2 mm in diameter. 6 weeks after treatment, tumor size (g) and spontaneous lung metastasis (h) was determined. The lung metastatic areas of the representative lungs were highlighted with red (h). Vehicle, n=10 mice; C26-A6, n=10 mice. Size bar, 5 mm. i-k, PyMT tumor cells with endogenous SND1 stably knockdown was confirmed by western blot (i). 10k of the cells were injected into FVB females and treated similarly as in (g, h). Tumor size (j) and lung metastasis (k) was assessed. The lung metastatic areas of the representative lungs were highlighted with red (k). Vehicle, n=10 mice; C26-A6, n=10 mice. Size bar, 5 mm. l,m, 2k of PyMT tumor cells were injected into FVB femalesvia tail-vein. 3 days after injection, the mice were treated with vehicle or C26-A6 (l). 5 weeks later, lung metastatic nodules were counted (m). n = 6 mice per group. Data represent mean ± SEM. Significance determined by Two-Way Repeated Measures ANOVA test (b) and two tailed Student’s t-test (c,d,j,h,k,m). AU: arbitrary units. Numerical source data for b-d, g, h, j, k, m and uncropped blots for f and i are provided.