TABLE 5.

Immobilized primers for on-chip PCR amplification

Oligonucleotidea	Amino acid position	Amino acid substitutionb	Nucleotide substitution	Sequence (5′→3)c	Sequence position
a1pcr	513	Gln (WT)	CAA	TTCGGCACCAGCCAGCCAGCTGAGCCA	2355–2378
b1pcr	513	Gln→Leu	CAA→CTA	TTCGGCACCAGCCAGCCAGCTGAGCCt	2355–2377
c1pcr	513	Gln→Lys	CAA→AAA	TTCGGCACCAGCCAGCCAGCTGAGCa	2355–2376
d1pcr	513	Gln→Pro	CAA→CCA	TTCGGCACCAGCCAGCCAGCTGAGCCc	2355–2377
a2pcr	515	Met (WT)	ATG	TCGGCACCAGCCAGCTGAGACAATTCATG	2356–2384
b2pcr	515	Met→Ile	ATG→ATA	TCGGCACCAGCCAGCTGAGACAATTCATa	2356–2384
a3pcr	516	Asp (WT)	GAC	GCACCAGCCAGCTGAGACAATTCATGGAC	2359–2387
b3pcr	516	Asp→Tyr	GAC→TAC	GCACCAGCCAGCTGAGCCAATTCATGt	2359–2385
c3pcr	516	Asp→Gly	GAC→GGC	GCACCAGCCAGCTGAGACAATTCATGGg	2359–2386
d3pcr	516	Asp→Val	GAC→GTC	GCACCAGCCAGCTGAGACAATTCATGGt	2359–2386
e3pcr	516	Asp→Glu	GAC→GAG	GCACCAGCCAGCTGAGACAATTCATGGAg	2359–2387
a4pcr	522	Ser (WT)	TCG	GCCAATTCATGGACCAGAACAACCCGCTGTC	2374–2404
b4pcr	522	Ser→Leu	TCG→TTG	GCCAATTCATGGACCAGAACAACCCGCTGTt	2374–2404
a5pcr	524	Leu (WT)	TTG→TCC	CATGGACCAGAACAACCCGCTGTCGAGTTG	2381–2410
b5pcr	524	Leu→Ser	TTG→TCC	CATGGACCAGAACAACCCGCTGTCGAGTTc	2381–2420
a6pcr	526	His (WT)	CAC	CCAGAACTACCCGCTGTCGTGGTTGACCC	2387–2415
b6pcr	526	His→Leu	CAC→CTC	CCAGAACTACCCGCTGTCGTGGTTGACCCt	2387–2416
c6pcr	526	His→Asp	CAC→GAC	CCAGAACTACCCGCTGTCGTGGTTGACCg	2387–2415
d6pcr	526	His→Gln2	CAC→CAA	CCAGAACTACCCGCTGTCGTGGTTGACCCAa	2387–2417
e6pcr	526	His→Gln	CAC→CAG	CCAGAACTACCCGCTGTCGTGGTTGACCCAg	2387–2417
f6pcr	526	His→Cys	CAC→TGC	CCAGAACTACCCGCTGTCGTGGTTGACCTg	2387–2416
g6pcr	526	His→Asn	CAC→AAC	CCAGAACTACCCGCTGTCGTGGTTGACCa	2387–2415
h6pcr	526	His→Arg	CAC→CGC	CCAGAACTACCCGCTGTCGTGGTTGACCCg	2387–2416
i6pcr	526	His→Pro	CAC→CCC	CCAGAACTACCCGCTGTCGTGGTTGACCCc	2387–2416
j6pcr	526	His→Tyr	CAC→TAC	CCAGAACTACCCGCTGTCGTGGTTGACCt	2387–2415
a7pcr	531	Ser (WT)	TCG	GGTTGACCAACAAGCGCCGACTGTC	2407–2431
b7pcr	531	Ser→Leu	TCG→TTG	GGTTGACCAACAAGCGCCGACTGTt	2407–2431
c7pcr	531	Ser→Trp	TCG→TGG	GGTTGACCAACAAGCGCCGACTGTGg	2407–2431
d7pcr	531	Ser→Gln	TCG→CAG	GGTTGACCAACAAGCGCCGACTGca	2407–2431
e7pcr	531	Ser→Cys	TCG→TGT	GGTTGACCAACAAGCGCCGACTGTGt	2407–2432
a8pcr	533	Leu (WT)	CTG	TGACCCACAAGCGCCGACTGTCGGAGCT	2413–2437
b8pcr	533	Leu→Pro	CTG→CCG	TGACCCACAAGCGCCGACTGTCGGAGc	2413–2437

^aThe designations of oligonucleotides correspond to their locations in Fig. 3. 

^bWT, wild type. 

^cThe sequences belong to the antisense strand. The variable 3′ nucleotides are shown in lowercase.