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. 2022 Feb 5;20:75. doi: 10.1186/s12967-022-03276-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Linc00261 regulates CSCs related markers’ expression in HCC cells. A, B Western blotting analysis (A) and immunofluorescence staining analysis (B) of CSC-markers (CD44 and CD133) and -transcription factors (SOX2 and OCT4) in SMMC-7721 cells after overexpression of linc00261 using lentivirus. The histogram represents quantitative analyses of the relative gray values of the western blotting bands. C Western blotting analysis of CSC-markers (CD44 and CD133) and CSC-transcription factors (SOX2 and OCT4) protein levels in Huh7 cells and MHCC-LM3 cells after transfection with control siRNA or siRNAs against linc00261. The histogram represents quantitative analyses of the relative gray values of western blotting bands. D Linc00261 overexpression inhibits sphere formation both numbers and size in SMMC-7721 cells.* P < 0.05; ** P < 0.01; *** P < 0.001