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. 2021 Aug 25;27(20):5669–5680. doi: 10.1158/1078-0432.CCR-21-0312

Figure 2.

Figure 2. The activity of TOP2B on promoter and introns regulates gene expression in BT142 glioma cell line. A, GSVA plot of gene expression [RNA-seq DMSO (five experimental replicates) and ICRF-193 in seven experimental replicates] with TOP2 inhibition at promoters with TOP2B peaks detected by DMSO ChIP only, TOP2B peaks detected by DMSO and etoposide (Et) ChIP, and TOP2B peaks only detected etoposide ChIP only. B, Examples of ChIP-seq data and RNA-seq at promoter sites. C, GSVA analysis of effect of TOP2 inhibition on genes with TOP2B native binding and/or activity on introns (presented similar to A). D, Examples of ChIP-seq data and RNA-seq at intron sites. For B and D, the y-scale is maintained constant for all tracks within a specific locus.

The activity of TOP2B on promoter and introns regulates gene expression in BT142 glioma cell line. A, GSVA plot of gene expression [RNA-seq DMSO (five experimental replicates) and ICRF-193 in seven experimental replicates] with TOP2 inhibition at promoters with TOP2B peaks detected by DMSO ChIP only, TOP2B peaks detected by DMSO and etoposide (Et) ChIP, and TOP2B peaks only detected etoposide ChIP only. B, Examples of ChIP-seq data and RNA-seq at promoter sites. C, GSVA analysis of effect of TOP2 inhibition on genes with TOP2B native binding and/or activity on introns (presented similar to A). D, Examples of ChIP-seq data and RNA-seq at intron sites. For B and D, the y-scale is maintained constant for all tracks within a specific locus.